| Gene | Gprin3 |
| Chromosome | 6 |
| Deletion Start | 59,355,317 |
| Deletion End | 59,353,009 |
| Deletion Size | 2,309 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,354,476 | 59,354,495 | CTGCTACCAGCACAGTATCC |
| TUP | 59,354,436 | 59,354,465 | TTTAAAGAAGCCAGTACAATGACCTGCCAA |
| TUR | 59,354,402 | 59,354,422 | CCCTGCCAGAGACCTCCTTAG |
| TDF | 59,353,581 | 59,353,601 | GGGAGGAAGAGCCAACAGAAC |
| TDP | 59,353,556 | 59,353,580 | ACCTTGGAAGAACTCAGGCAGCCCA |
| TDR | 59,353,509 | 59,353,526 | CGAGTCACCTGTGCCATC |
PCR Primers
| Primer | Sequence |
| SU | GAGTCTCACTGCCTTGTCCAG |
| SD | CCATCCTGTGATAGACTGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11150A-B8 | VGB6 | C57BL/6NTac | |
| 11150A-H11 | VGB6 | C57BL/6NTac | |
| 11150A-H5 | VGB6 | C57BL/6NTac | |
| 11150A-C8 | VGB6 | C57BL/6NTac | |
| 11150A-C5 | VGB6 | C57BL/6NTac | |
