| Gene | Irgq |
| Chromosome | 7 |
| Deletion Start | 24,531,389 |
| Deletion End | 24,534,570 |
| Deletion Size | 3,201 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,532,072 | 24,532,092 | ACCACACCCATGAGCACAGAG |
| TUP | 24,532,095 | 24,532,122 | TGAGATCATGGAGACTCTGCCATAAGCA |
| TUR | 24,532,163 | 24,532,181 | GGTGGGCTTTCCAGTTGAC |
| TDF | 24,534,378 | 24,534,397 | CAACAGGTGGGCTGGGTTAC |
| TDP | 24,534,398 | 24,534,422 | CGTGCTGCGCACGGTGTACTTCTAC |
| TDR | 24,534,431 | 24,534,450 | GCATCAGCCAGCATCTCATC |
PCR Primers
| Primer | Sequence |
| SU | ACCATTCTCGAGTTTGGCAG |
| SD | TCCTGCCACCTAAGAAATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 587 bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 474 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11151A-A7 | VGB6 | C57BL/6NTac | |
| 11151A-E1 | VGB6 | C57BL/6NTac | |
| 11151A-F2 | VGB6 | C57BL/6NTac | |
| 11151A-G5 | VGB6 | C57BL/6NTac | |
| 11151A-G6 | VGB6 | C57BL/6NTac | |
| 11151A-G12 | VGB6 | C57BL/6NTac | |
