| Gene | Bcl2a1c |
| Chromosome | 9 |
| Deletion Start | 114,330,141 |
| Deletion End | 114,331,020 |
| Deletion Size | 880 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 114,330,238 | 114,330,256 | CGGCTCCAAGCCAAGCATT |
| TUP | 114,330,257 | 114,330,286 | CAGAGTGCTACAAAGAGTTGCTTTCTCCGT |
| TUR | 114,330,288 | 114,330,312 | TTAGGTTCTTTCCAACTTCCTTCTG |
| TDF | 114,330,694 | 114,330,715 | GGACTTCACTCTGTTTGTATGC |
| TDP | 114,330,721 | 114,330,744 | TTTTAGATACTCTGAGTTAAAATT |
| TDR | 114,330,766 | 114,330,789 | CAGTAGAAGCCATTGGAAATGACC |
PCR Primers
| Primer | Sequence |
| SU | GAGGAAGTGGTGTGTTGCTC |
| SD | TAGCTACACATGATAGTGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 352 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 370 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11155A-B3 | VGB6 | C57BL/6NTac | |
| 11155A-C8 | VGB6 | C57BL/6NTac | |
