| Gene | Pfn3 |
| Chromosome | 13 |
| Deletion Start | 55,415,163 |
| Deletion End | 55,414,734 |
| Deletion Size | 430 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,415,141 | 55,415,161 | TGACTGGAAGGGCTACATCAG |
| TUP | 55,415,116 | 55,415,138 | CAGTGCTGCGGGATCAGCGGATC |
| TUR | 55,415,067 | 55,415,086 | ATGCCCACACACAGCGATTG |
| TDF | 55,414,827 | 55,414,845 | CTGGTGCTCATGGGCAGAC |
| TDP | 55,414,801 | 55,414,825 | AGGCGTGCATGGAGGCATCCTCAAT |
| TDR | 55,414,766 | 55,414,783 | TCACGCAGCCCACCAATC |
PCR Primers
| Primer | Sequence |
| SU | GCTCTGTGTAATGAATGCTC |
| SD | AATATTCCTAATCAGGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 398 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 348 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11158A-E2 | VGB6 | C57BL/6NTac | |
| 11158A -A10 | VGB6 | C57BL/6NTac | |
