| Gene | Ppp1r3d |
| Chromosome | 2 |
| Deletion Start | 178,414,204 |
| Deletion End | 178,413,368 |
| Deletion Size | 837 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 178,413,848 | 178,413,868 | CGCCTAGCCATCAACTCAGAC |
| TUP | 178,413,818 | 178,413,841 | TGCAGCAGCCAGGATCTGGAGTTC |
| TUR | 178,413,787 | 178,413,805 | GCGAGAAGTCGGGAACCAG |
| TDF | 178,413,503 | 178,413,521 | CGGCTCCCAGGTACACTTC |
| TDP | 178,413,479 | 178,413,501 | CACTGCGCTATGGCGTAGCTGGT |
| TDR | 178,413,416 | 178,413,435 | CAAGGCATGACTGCGACAAG |
PCR Primers
| Primer | Sequence |
| SU | GTTGCGCTTGGAGAAGTTGC |
| SD | GCAAAACACTGACCTGGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 420 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 378 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11161A-A4 | VGB6 | C57BL/6NTac | |
| 11161A-B10 | VGB6 | C57BL/6NTac | |
| 11161A-E9 | VGB6 | C57BL/6NTac | |
| 11161A-E11 | VGB6 | C57BL/6NTac | |
| 11161A-E10 | VGB6 | C57BL/6NTac | |
| 11161A-E2 | VGB6 | C57BL/6NTac | |
