| Gene | Neu4 |
| Chromosome | 1 |
| Deletion Start | 94,022,393 |
| Deletion End | 94,025,488 |
| Deletion Size | 3,096 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,022,725 | 94,022,742 | ACTGGAGGAGCACAGGTC |
| TUP | 94,022,743 | 94,022,765 | TATGAACCCTTGCCCGGTGCTGG |
| TUR | 94,022,789 | 94,022,809 | CAGCACGGCAATGAAGAAGAG |
| TDF | 94,024,690 | 94,024,708 | TCGAAGCCCTCTGGGTAAC |
| TDP | 94,024,713 | 94,024,735 | TGCAGGCACTGAGTGCTGATGAA |
| TDR | 94,024,770 | 94,024,787 | GAGCCGTCTCTGCCAATG |
PCR Primers
| Primer | Sequence |
| SU | GCTGAGGCAGATAGAAAGCG |
| SD | TCAGCATGTTTCAAAGTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11162A-D10 | VGB6 | C57BL/6NTac | |
| 11162A-F3 | VGB6 | C57BL/6NTac | |
| 11162A-F10 | VGB6 | C57BL/6NTac | |
