| Gene | Ndufb2 |
| Chromosome | 6 |
| Deletion Start | 39,592,618 |
| Deletion End | 39,598,607 |
| Deletion Size | 5,990 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 39,592,955 | 39,592,975 | AATCCAGTCCCGCACAGTCTC |
| TUP | 39,592,977 | 39,592,999 | CGGACTGAGCCTTCCAAGCAGCT |
| TUR | 39,593,035 | 39,593,056 | GTCTTGTCTCTGAGCCCATCTC |
| TDF | 39,597,842 | 39,597,866 | GCATTTCCGTTGTATTAGGCTGTAG |
| TDP | 39,597,875 | 39,597,899 | TGATATTGCAGCAGACAGGGCTCCA |
| TDR | 39,597,906 | 39,597,928 | TCTCAAGTGGATGCAGTCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | GCAAGAACATGCATGAAAGC |
| SD | GGTCCTCTTGTCTCCGCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 420 bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 425 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11163A-A6 | VGB6 | C57BL/6NTac | |
| 11163A-C12 | VGB6 | C57BL/6NTac | |
| 11163A-D12 | VGB6 | C57BL/6NTac | |
| 11163A-H7 | VGB6 | C57BL/6NTac | |
