| Gene | 4930563E22Rik |
| Chromosome | 11 |
| Deletion Start | 72,215,324 |
| Deletion End | 72,215,725 |
| Deletion Size | 402 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,215,384 | 72,215,402 | AAGCACCCGCCACAAGGAG |
| TUP | 72,215,404 | 72,215,426 | CATCGACTGTCCGCGTGGAGACG |
| TUR | 72,215,442 | 72,215,461 | ACGCGGTGGGATGACGTTTC |
| TDF | 72,215,551 | 72,215,570 | CGAAGCGACTCCCTCAGTTC |
| TDP | 72,215,572 | 72,215,593 | TTGAGGTGTCCTCCCAGCACGT |
| TDR | 72,215,677 | 72,215,694 | TGGCTGGGCTCTCAACAC |
PCR Primers
| Primer | Sequence |
| SU | TTGGTGAGAATCCGGAAGCC |
| SD | CTTACCTGGTGTAGAGACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 415 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 144 bp | + | + | - |
| NeoFwd | SD | 363 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11164B-H6 | VGB6 | C57BL/6NTac | |
| 11164C-G3 | VGB6 | C57BL/6NTac | |
