| Gene | Tmem102 |
| Chromosome | 11 |
| Deletion Start | 69,805,230 |
| Deletion End | 69,803,593 |
| Deletion Size | 1,638 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,805,150 | 69,805,172 | GGCCTCTCACAGACATAGATTTC |
| TUP | 69,805,122 | 69,805,141 | CGCCCAGCTGCAGGAGCTCA |
| TUR | 69,805,079 | 69,805,100 | TCACTCCAACTCTCCTGAACAC |
| TDF | 69,804,342 | 69,804,360 | TATGCCCGACCCAAGTAAC |
| TDP | 69,804,313 | 69,804,340 | TCCTGGTTCTTCGTTAAGTTGGCTGAGG |
| TDR | 69,804,253 | 69,804,271 | CGTGACGAGCTGCATGAAC |
PCR Primers
| Primer | Sequence |
| SU | ATTGTATTCCCAGGAGGGAC |
| SD | GGTATGAGCCAATTGATCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 393 bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | 395 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11165B-E7 | VGB6 | C57BL/6NTac | |
| 11165B-H11 | VGB6 | C57BL/6NTac | |
| 11165D-H5 | VGB6 | C57BL/6NTac | |
| 11165D-F7 | VGB6 | C57BL/6NTac | |
| 11165C-D6 | VGB6 | C57BL/6NTac | |
