| Gene | Mcts2 |
| Chromosome | 2 |
| Deletion Start | 152,687,274 |
| Deletion End | 152,687,820 |
| Deletion Size | 547 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 152,687,346 | 152,687,366 | AAGAGCCAACTGACTGAGCAG |
| TUP | 152,687,369 | 152,687,391 | TCCAGGTATCGAGCCGTGGCTTA |
| TUR | 152,687,442 | 152,687,466 | CGTTGACTGTAAGGATTTCCATGTG |
| TDF | 152,687,585 | 152,687,608 | GCTCAGTGGTGCAAATATCATGTG |
| TDP | 152,687,610 | 152,687,633 | CCGGGTTTAACGTCTCCTGGAGCG |
| TDR | 152,687,655 | 152,687,673 | TGACCGCCACGATGGTATC |
PCR Primers
| Primer | Sequence |
| SU | TTGCAACCGGAAGTCCGGAG |
| SD | AGGAGCACCTTGGTAGAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 406 bp | - | + | + |
| TUF | TUR | 121 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 397 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11167C-B12 | VGB6 | C57BL/6NTac | |
| 11167C-A10 | VGB6 | C57BL/6NTac | |
