| Gene | Cd70 |
| Chromosome | 17 |
| Deletion Start | 57,149,598 |
| Deletion End | 57,146,072 |
| Deletion Size | 3,527 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,149,096 | 57,149,114 | AGCCGCCTGTGGCTGAAAG |
| TUP | 57,149,074 | 57,149,094 | CCAGGCACCGCCCACATGCAT |
| TUR | 57,149,045 | 57,149,066 | ATGCCACCCTTCCCACTACTTC |
| TDF | 57,146,792 | 57,146,809 | GTGAGACTTGGGCTGGAC |
| TDP | 57,146,771 | 57,146,791 | TCAGAGGCAGCTCCGGGCATC |
| TDR | 57,146,701 | 57,146,721 | CAGGACATGGTGCCTGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | CTGAATGCCCAATCGGAAGC |
| SD | GCCTGAAACCCATCAGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 384 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | 237 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11171A-C7 | VGB6 | C57BL/6NTac | |
| 11171A-H3 | VGB6 | C57BL/6NTac | |
