| Gene | Fam179a |
| Chromosome | 17 |
| Deletion Start | 71,716,272 |
| Deletion End | 71,729,525 |
| Deletion Size | 13,254 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,716,917 | 71,716,937 | CTAGCTGTGCGATCTGAGGAG |
| TUP | 71,716,940 | 71,716,962 | TCAGTCTCCTCCATGAGCCCTCC |
| TUR | 71,716,982 | 71,717,001 | TGCTGGTGCCGTTATCATTC |
| TDF | 71,728,855 | 71,728,873 | TGGGATTGTGACCCACCTC |
| TDP | 71,728,875 | 71,728,897 | TTCCTCCTCACTGGTCCGAGGGA |
| TDR | 71,728,926 | 71,728,946 | ACCGCAAAGCCAATGCAAATG |
PCR Primers
| Primer | Sequence |
| SU | TCTCCCTCGACACTTGCAC |
| SD | AAACTAGGTTCAAGGTTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11177B-C6 | VGB6 | C57BL/6NTac | |
| 11177B-E2 | VGB6 | C57BL/6NTac | |
| 11177C-F4 | VGB6 | C57BL/6NTac | |
| 11177C-H2 | VGB6 | C57BL/6NTac | |
| 11177C-F5 | VGB6 | C57BL/6NTac | |
| 11177B-B12 | VGB6 | C57BL/6NTac | |
