| Gene | Tmem92 |
| Chromosome | 11 |
| Deletion Start | 94,781,274 |
| Deletion End | 94,777,488 |
| Deletion Size | 3,787 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,782,017 | 94,782,038 | GGTCTCTCCTTCCCTGTTCCTC |
| TUP | 94,781,982 | 94,782,010 | TGGTGACCTTGGGTGTAGTTTATTGTCCC |
| TUR | 94,781,942 | 94,781,965 | GAACTTCCACTTCTCCGGTTACAC |
| TDF | 94,779,018 | 94,779,040 | CTGCACTGAATTACAGCACGATG |
| TDP | 94,778,979 | 94,779,008 | TGGATCATCAGACTCCTATAGAACCGCCCT |
| TDR | 94,778,903 | 94,778,923 | TCAGAAAGACCCACCTGACTG |
PCR Primers
| Primer | Sequence |
| SU | GTGCTCTCCTCCATCCAAC |
| SD | CCCACTAGCAATGATGTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 138 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11179A-F9 | VGB6 | C57BL/6NTac | |
| 11179A-E6 | VGB6 | C57BL/6NTac | |
| 11179A-D10 | VGB6 | C57BL/6NTac | |
| 11179A-B12 | VGB6 | C57BL/6NTac | |
