| Gene | Igsf5 |
| Chromosome | 16 |
| Deletion Start | 96,364,030 |
| Deletion End | 96,403,389 |
| Deletion Size | 39,360 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,364,829 | 96,364,847 | CCACTGCCTCTGGCTTCTC |
| TUP | 96,364,850 | 96,364,875 | TTCTCTGTGTCTCCCATGATGGCAAC |
| TUR | 96,364,891 | 96,364,912 | GGCCTGCAAATGTCTGTGTATC |
| TDF | 96,402,435 | 96,402,457 | TTTCTTTCCTGATTGGGAGTCTG |
| TDP | 96,402,459 | 96,402,483 | AAATGAAAGCCACACGGCAGCTTGA |
| TDR | 96,402,493 | 96,402,516 | GTGGAATGTTGTGGATCTTGAGTG |
PCR Primers
| Primer | Sequence |
| SU | TTCAGTGTGCATGCTAAGGG |
| SD | GAGCTCCAGCTGCCAGTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11181A-E5 | VGB6 | C57BL/6NTac | |
| 11181B-B7 | VGB6 | C57BL/6NTac | |
