| Gene | Lypd4 |
| Chromosome | 7 |
| Deletion Start | 24,867,291 |
| Deletion End | 24,864,640 |
| Deletion Size | 2,652 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,866,697 | 24,866,716 | GCGAGGAGACCGTAGTGTTC |
| TUP | 24,866,668 | 24,866,695 | TCGAGACGGGTGATCCTTCTGTTCATTC |
| TUR | 24,866,640 | 24,866,662 | GACTAAGAAGGCAGGCAGGAGAG |
| TDF | 24,865,158 | 24,865,177 | GCAGGTGAGAGCAGATGAAC |
| TDP | 24,865,130 | 24,865,155 | TTGTCAAGTGACTGTCTGTGGGCAGT |
| TDR | 24,865,106 | 24,865,124 | GAACAAAGGCCCTGGAGAC |
PCR Primers
| Primer | Sequence |
| SU | AGCCTGTGTAATGGTGGGTC |
| SD | TCCCTTCCAAAGCATCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 441 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 374 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11182B-A1 | VGB6 | C57BL/6NTac | |
| 11182B-A11 | VGB6 | C57BL/6NTac | |
| 11182B-D3 | VGB6 | C57BL/6NTac | |
| 11182B-F2 | VGB6 | C57BL/6NTac | |
| 11182B-G11 | VGB6 | C57BL/6NTac | |
| 11182B-D10 | VGB6 | C57BL/6NTac | |
