| Gene | Pfn2 |
| Chromosome | 3 |
| Deletion Start | 57,847,479 |
| Deletion End | 57,843,198 |
| Deletion Size | 4,282 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,847,453 | 57,847,473 | TGGCAGAGCTACGTGGATAAC |
| TUP | 57,847,431 | 57,847,452 | CTGATGTGCGATGGCTGCTGCC |
| TUR | 57,847,381 | 57,847,399 | GGCTGCCCAGACGTATTTG |
| TDF | 57,843,596 | 57,843,615 | ATCAGCCAGACTGTTTCTCC |
| TDP | 57,843,561 | 57,843,590 | TGCAAGGTAAACTGTCACTTTCCTCCTAGC |
| TDR | 57,843,503 | 57,843,523 | TGCATGTAGGAAACCCCACTG |
PCR Primers
| Primer | Sequence |
| SU | TGATTGTCCCCGGCGGTATC |
| SD | TTGTTAATTGTGCCTGCGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 523 bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 113 bp | + | + | - |
| NeoFwd | SD | 303 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11185A-D5 | VGB6 | C57BL/6NTac | |
| 11185B-B5 | VGB6 | C57BL/6NTac | |
| 11185B-B10 | VGB6 | C57BL/6NTac | |
