| Gene | Irx1 |
| Chromosome | 13 |
| Deletion Start | 71,963,485 |
| Deletion End | 71,958,506 |
| Deletion Size | 4,980 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,963,113 | 71,963,133 | TGGCCCAACAGAGAAACAGAG |
| TUP | 71,963,089 | 71,963,110 | TCCGCAGGCAGTGTCTGGGAAA |
| TUR | 71,963,069 | 71,963,087 | TGCAGGACCCTTCTCGTTC |
| TDF | 71,959,109 | 71,959,128 | CCGAGGCACTGAGCTGTATC |
| TDP | 71,959,068 | 71,959,097 | TCCACGGAGGTATAAGGGTAGACATTGGTC |
| TDR | 71,959,023 | 71,959,047 | TGTTCAGGTTGGAAGGGTTTCTATG |
PCR Primers
| Primer | Sequence |
| SU | CTTCAAATTGTGTCTGAGAGC |
| SD | CTGAAATCACGCTATATACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 400 bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 303 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11187B-D12 | VGB6 | C57BL/6NTac | |
| 11187B-E2 | VGB6 | C57BL/6NTac | |
| 11187B-F4 | VGB6 | C57BL/6NTac | |
| 11187B-D3 | VGB6 | C57BL/6NTac | |
