| Gene | Kctd4 |
| Chromosome | 14 |
| Deletion Start | 75,962,572 |
| Deletion End | 75,963,373 |
| Deletion Size | 802 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,962,629 | 75,962,649 | GTACGAAGGGAGGCACAACAG |
| TUP | 75,962,652 | 75,962,673 | TGGAAGATGCCGAGCAAGGCAA |
| TUR | 75,962,674 | 75,962,693 | TCAGCGTGGATTTGCAGTTC |
| TDF | 75,963,286 | 75,963,309 | AAAATGTGGGTTTAGACTGCTGAC |
| TDP | 75,963,311 | 75,963,333 | AGCCTGGACTGCTCCAAAGGGTC |
| TDR | 75,963,338 | 75,963,361 | AAAATGAAGTGCATCGCTGTGAAC |
PCR Primers
| Primer | Sequence |
| SU | TGCAACAAATCCCTTCCCTG |
| SD | TTACAGTTTAGAAGACCACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 366 bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 305 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11191A-A1 | VGB6 | C57BL/6NTac | |
| 11191B-C5 | VGB6 | C57BL/6NTac | |
| 11191B-D7 | VGB6 | C57BL/6NTac | |
| 11191B-C10 | VGB6 | C57BL/6NTac | |
| 11191C-H3 | VGB6 | C57BL/6NTac | |
| 11191C-B4 | VGB6 | C57BL/6NTac | |
