| Gene | Ccdc87 |
| Chromosome | 19 |
| Deletion Start | 4,839,466 |
| Deletion End | 4,842,088 |
| Deletion Size | 2,623 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,840,117 | 4,840,136 | GCCTCACAGCACTGGCTTAG |
| TUP | 4,840,138 | 4,840,161 | CCACATGCCGTGCTCTAGCCTCAA |
| TUR | 4,840,175 | 4,840,194 | TATGGGCGGCTGAGTTGGAC |
| TDF | 4,841,208 | 4,841,226 | GGGACAAGCGACCAAACAG |
| TDP | 4,841,230 | 4,841,257 | CGGTGTATGGATGAATTGGCTGAAACCC |
| TDR | 4,841,293 | 4,841,315 | GGAGGAAATCCGACTCCTGAAAG |
PCR Primers
| Primer | Sequence |
| SU | TCCACATGAAGCTTTCACTC |
| SD | AACAAGAGTGATTTGGCACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 423 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | 338 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11192A-G3 | VGB6 | C57BL/6NTac | |
| 11192A-F3 | VGB6 | C57BL/6NTac | |
| 11192A-H2 | VGB6 | C57BL/6NTac | |
