| Gene | Trappc2 |
| Chromosome | X |
| Deletion Start | 166,446,813 |
| Deletion End | 166,452,242 |
| Deletion Size | 5,430 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 166,447,653 | 166,447,674 | ACTCCCATGTTGTCATCCTGTC |
| TUP | 166,447,692 | 166,447,718 | TTATTTCTGTGGCTTTTCCAGCACTGC |
| TUR | 166,447,734 | 166,447,752 | TGGCCTGGCTGTCAGTAAG |
| TDF | 166,451,759 | 166,451,782 | CATGAACTCACTGTGCAGCTAAAG |
| TDP | 166,451,785 | 166,451,810 | AGGCTTGAATTCCTGGTCCTCCTGAA |
| TDR | 166,451,822 | 166,451,843 | GCTTAGCCAGGTGCAATTTCTG |
PCR Primers
| Primer | Sequence |
| SU | GATGAAGGGACCCTTGAGC |
| SD | AGCTTCTTGGTACTGCAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 440 bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 482 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11197A-C12 | VGB6 | C57BL/6NTac | |
| 11197A-F3 | VGB6 | C57BL/6NTac | |
| 11197B-E5 | VGB6 | C57BL/6NTac | |
| 11197B-H7 | VGB6 | C57BL/6NTac | |
| 11197C-A1 | VGB6 | C57BL/6NTac | |
