| Gene | Otud6a |
| Chromosome | X |
| Deletion Start | 100,428,996 |
| Deletion End | 100,429,923 |
| Deletion Size | 928 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,429,106 | 100,429,126 | TCCGTCCCAAAGAACGACAAG |
| TUP | 100,429,132 | 100,429,155 | ACGGAAGCAGATGCTCGCGGACAT |
| TUR | 100,429,172 | 100,429,190 | TGTGCCTCTGCTCCATCTC |
| TDF | 100,429,728 | 100,429,750 | CCAACTCACCCACCATTGTCATC |
| TDP | 100,429,755 | 100,429,779 | AGGAGTACACCAGGAAGCCGGTCAC |
| TDR | 100,429,784 | 100,429,804 | GCAGGCGTAGTGCAGATAGAC |
PCR Primers
| Primer | Sequence |
| SU | CATGCTACGGGGACCCAAC |
| SD | GCCACATGAAAGGAGCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 369 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 391 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11202B-A9 | VGB6 | C57BL/6NTac | |
| 11202B-B9 | VGB6 | C57BL/6NTac | |
| 11202B-C2 | VGB6 | C57BL/6NTac | |
| 11202B-G12 | VGB6 | C57BL/6NTac | |
| 11202C-A1 | VGB6 | C57BL/6NTac | |
| 11202C-F2 | VGB6 | C57BL/6NTac | |
