| Gene | Rrs1 |
| Chromosome | 1 |
| Deletion Start | 9,545,508 |
| Deletion End | 9,546,621 |
| Deletion Size | 1,114 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 9,545,985 | 9,546,004 | GCACCTCGATCAGCCATTCT |
| TUP | 9,545,958 | 9,545,978 | TCATCCCGGGCGCGCTTGTAG |
| TUR | 9,545,884 | 9,545,904 | AAGGGAATCCGTCCCAAGAAG |
| TDF | 9,546,149 | 9,546,171 | CTACTGGACACCAGAGTAAGGAA |
| TDP | 9,546,175 | 9,546,193 | CTGGGCCGCGCCATGCAAG |
| TDR | 9,546,197 | 9,546,216 | CGAAGCGGTGGAAACCTTGG |
PCR Primers
| Primer | Sequence |
| SU | GCATGCTGGGAAACGTAGTC |
| SD | ATGTTGAAACCAATCTACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 425 bp | - | + | + |
| TUF | TUR | 121 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 356 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11204A-C6 | VGB6 | C57BL/6NTac | |
| 11204A-E2 | VGB6 | C57BL/6NTac | |
| 11204A-G6 | VGB6 | C57BL/6NTac | |
