| Gene | Sval2 |
| Chromosome | 6 |
| Deletion Start | 41,860,342 |
| Deletion End | 41,864,413 |
| Deletion Size | 4,072 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,860,404 | 41,860,426 | TTTGGAGACTCCTTATGGTCAGG |
| TUP | 41,860,437 | 41,860,466 | AGGCCTTCTCTTTTCTGCATCACCTTCCTA |
| TUR | 41,860,467 | 41,860,488 | TCACCAACTGGGAAGACATTTC |
| TDF | 41,863,825 | 41,863,844 | TCTGCTCACGCCATTTACAC |
| TDP | 41,863,846 | 41,863,872 | TCCTGCCTTTGCAGTGCAAACAAATAC |
| TDR | 41,863,941 | 41,863,964 | CCACTCGAATCCATCCTGACAATC |
PCR Primers
| Primer | Sequence |
| SU | ATCAGGGAGGTGAGAGCTTG |
| SD | TTTCAATATTACTCTGCCATCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 140 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11208A-C5 | VGB6 | C57BL/6NTac | |
| 11208A-D1 | VGB6 | C57BL/6NTac | |
| 11208A-D2 | VGB6 | C57BL/6NTac | |
| 11208A-D5 | VGB6 | C57BL/6NTac | |
| 11208A-E6 | VGB6 | C57BL/6NTac | |
| 11208A-H1 | VGB6 | C57BL/6NTac | |
