| Gene | Snx33 |
| Chromosome | 9 |
| Deletion Start | 56,926,780 |
| Deletion End | 56,918,432 |
| Deletion Size | 8,349 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,925,851 | 56,925,870 | AGCGCAAGCGAAGGCTCATC |
| TUP | 56,925,825 | 56,925,848 | CTGGATGGACCACATGACCAGCCA |
| TUR | 56,925,791 | 56,925,811 | GTGCTGGAAGCCCTCATACTG |
| TDF | 56,918,696 | 56,918,713 | TCCTGCCAGGTGCCTTTG |
| TDP | 56,918,672 | 56,918,694 | CAAGGTGAAGGAAAGCCAGCGCA |
| TDR | 56,918,648 | 56,918,666 | GAGCCATTCGGCCCTCATC |
PCR Primers
| Primer | Sequence |
| SU | GGAGAGACCCGCTCCTTTCC |
| SD | GGAGTCCACGGAATGAACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 419 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | 384 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11209A-C8 | VGB6 | C57BL/6NTac | |
| 11209A-D6 | VGB6 | C57BL/6NTac | |
| 11209A-F1 | VGB6 | C57BL/6NTac | |
| 11209A-H4 | VGB6 | C57BL/6NTac | |
| 11209A-G3 | VGB6 | C57BL/6NTac | |
