| Gene | Armcx6 |
| Chromosome | X |
| Deletion Start | 134,750,077 |
| Deletion End | 134,749,155 |
| Deletion Size | 923 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,750,013 | 134,750,032 | GGAGCTGGTGCCTGTTACTG |
| TUP | 134,749,980 | 134,750,007 | ACAAACTGACCATGGGAAGAAGTGAGGG |
| TUR | 134,749,923 | 134,749,945 | TCATCCAGATCCTGTCCATCTTC |
| TDF | 134,749,699 | 134,749,723 | TCCTTCACTTGTATTCTTGACCTGA |
| TDP | 134,749,676 | 134,749,697 | CAAGTGTGTTTCCACTCAAAGA |
| TDR | 134,749,636 | 134,749,658 | GGGAAAAGCCAGCATTTATGTTC |
PCR Primers
| Primer | Sequence |
| SU | GGTGGGTAGGAGGAGGAGAG |
| SD | GACAGGGAGATCACCACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11210C-C12 | VGB6 | C57BL/6NTac | |
| 11210C-E7 | VGB6 | C57BL/6NTac | |
| 11210C-G2 | VGB6 | C57BL/6NTac | |
| 11210C-H5 | VGB6 | C57BL/6NTac | |
