| Gene | Abpb |
| Chromosome | 7 |
| Deletion Start | 34,013,863 |
| Deletion End | 34,011,841 |
| Deletion Size | 2,023 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,013,149 | 34,013,170 | AACGTCCTCCCTAATCCCACTC |
| TUP | 34,013,121 | 34,013,146 | CCATGCAGCAAATGGTGGAAGGCTTT |
| TUR | 34,013,051 | 34,013,070 | GGGCAAGGGTTTGACTACTC |
| TDF | 34,012,775 | 34,012,793 | CACTTCCACTGGCCTCATC |
| TDP | 34,012,750 | 34,012,774 | TGAGCTACAGTCTGAACCGCCCTTA |
| TDR | 34,012,703 | 34,012,721 | AGAACAGCCACAGGCACTC |
PCR Primers
| Primer | Sequence |
| SU | GGGCTGCTGACAAAGGTTAG |
| SD | GACAACACATGAGGTGAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11211B-A1 | VGB6 | C57BL/6NTac | |
| 11211B-H5 | VGB6 | C57BL/6NTac | |
| 11211B-H3 | VGB6 | C57BL/6NTac | |
| 11211B-F4 | VGB6 | C57BL/6NTac | |
| 11211B-G5 | VGB6 | C57BL/6NTac | |
