| Gene | Nat8l |
| Chromosome | 5 |
| Deletion Start | 33,996,847 |
| Deletion End | 34,001,223 |
| Deletion Size | 4,377 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,997,653 | 33,997,676 | GCTAATTTATGACGGGAAGACAGC |
| TUP | 33,997,690 | 33,997,712 | AGCCAGGGAGACTGTTCGCCAGA |
| TUR | 33,997,730 | 33,997,751 | GCTTCAGACAGCAGAGGGTATC |
| TDF | 34,000,943 | 34,000,961 | TGCCATGCTGCACAACTAC |
| TDP | 34,000,962 | 34,000,983 | TCTGCAGTGGTACTGGGCACCA |
| TDR | 34,001,030 | 34,001,048 | ACTCGCGCCCATGTGTCTG |
PCR Primers
| Primer | Sequence |
| SU | TGAGGACCACTTCCTCGCA |
| SD | CTAGTCCTTCCCTGATGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 539 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 361 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11213A-C8 | VGB6 | C57BL/6NTac | |
| 11213A-D5 | VGB6 | C57BL/6NTac | |
| 11213A-F7 | VGB6 | C57BL/6NTac | |
