| Gene | Klhdc7a |
| Chromosome | 4 |
| Deletion Start | 139,967,631 |
| Deletion End | 139,965,306 |
| Deletion Size | 2,326 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,967,230 | 139,967,250 | CAGTGTCCAGACTCTCAGCAG |
| TUP | 139,967,209 | 139,967,228 | TGCCTCCTCCCTGTGGTGGC |
| TUR | 139,967,172 | 139,967,193 | GGTTGGGCTTACCTCTAACATC |
| TDF | 139,965,537 | 139,965,555 | AGCACCCGGCTCTGGTATG |
| TDP | 139,965,507 | 139,965,532 | TGCCACATACCGCTTACCTTACCCTG |
| TDR | 139,965,485 | 139,965,503 | ACAACGGCGCACTGGAAGG |
PCR Primers
| Primer | Sequence |
| SU | GCAGGTGACTGATCTGAAGC |
| SD | GTTGTGAGATTCCAGCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 394 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 313 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11217A-E2 | VGB6 | C57BL/6NTac | |
| 11217A-F1 | VGB6 | C57BL/6NTac | |
| 11217A-F4 | VGB6 | C57BL/6NTac | |
| 11217A-E7 | VGB6 | C57BL/6NTac | |
