| Gene | Ldoc1 |
| Chromosome | X |
| Deletion Start | 61,709,694 |
| Deletion End | 61,710,231 |
| Deletion Size | 538 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,709,827 | 61,709,845 | CTGCTGCGCCAGGTATGTC |
| TUP | 61,709,848 | 61,709,867 | CCGAGCTGTCCGGTGCCCTA |
| TUR | 61,709,940 | 61,709,962 | CATTGCAGAAGTGCTCCTCATTC |
| TDF | 61,710,010 | 61,710,030 | GAGGAGTGGGTGATGCCTTAC |
| TDP | 61,710,032 | 61,710,059 | TCGAGTCAAATAGCTACGTCCTCGGTGA |
| TDR | 61,710,084 | 61,710,105 | ACCCCAGCCAAAATACTGCTTC |
PCR Primers
| Primer | Sequence |
| SU | CAGCCACACCTTCCTTACC |
| SD | TGAGTTCCAGTGGATGTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 334 bp | - | + | + |
| TUF | TUR | 136 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 340 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11218A-E8 | VGB6 | C57BL/6NTac | |
| 11218A-F8 | VGB6 | C57BL/6NTac | |
