11222   Nap1l3     Status: ES cell colonies screened / QC positives

GeneNap1l3
ChromosomeX
Deletion Start122,397,016
Deletion End122,395,276
Deletion Size1,741
Genome Build38
MGI IDMGI:1859565
NCBI Gene ID54561
BAC ID461F18
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF122,396,750122,396,774CAGTCATTTGAACCGAAAGAAGAGG
TUP122,396,724122,396,745CTGAGCCTTCCAGAAGGGCCCA
TUR122,396,663122,396,684CACCCGATTTCTTACGGCTTGG
TDF122,395,633122,395,653GGAGTCGCCCTGGTATTACAG
TDP122,395,600122,395,625TGAAGTCCAGCCAAGAGTGGTTCCTA
TDR122,395,539122,395,560GGTTCCAGCTTCCCAATCAAAG

PCR Primers

PrimerSequence
SUACTGCCACAGCTGCTGCAG
SDGTACAATCTAATAGCAACATGAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev329 bp-++
TUFTUR112 bp++-
TDFTDR115 bp++-
NeoFwdSD310 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11222A-A7VGB6C57BL/6NTac
11222A-F12VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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