| Gene | Kcnk6 |
| Chromosome | 7 |
| Deletion Start | 29,232,480 |
| Deletion End | 29,224,986 |
| Deletion Size | 7,495 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,232,146 | 29,232,167 | CCGTGGGTAAGTGAGTGCTATC |
| TUP | 29,232,119 | 29,232,143 | TCACCCAGTCCCAGATAGTGTCCGG |
| TUR | 29,232,085 | 29,232,109 | AGGACGATTGGGATGAAGGTGTATG |
| TDF | 29,225,210 | 29,225,229 | TGCTCGTGTTGCAGACTTTC |
| TDP | 29,225,187 | 29,225,209 | CGCCGTGTTTCCGACCTCCATGG |
| TDR | 29,225,164 | 29,225,185 | GCAGCAAGATGAGTTCTGTGAG |
PCR Primers
| Primer | Sequence |
| SU | AACAGAAAGCTCCCACTTCG |
| SD | AACTAGGTGTGCCGGATGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11225A-H2 | VGB6 | C57BL/6NTac | |
| 11225A-E11 | VGB6 | C57BL/6NTac | |
| 11225A-D3 | VGB6 | C57BL/6NTac | |
