Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

11230 Zdhhc23 Status: ES cell colonies screened / QC positives

Gene	Zdhhc23
Chromosome	16
Deletion Start	43,979,047
Deletion End	43,969,125
Deletion Size	9,923
Genome Build	38

MGI ID	MGI:2685625
NCBI Gene ID	332175
BAC ID	273D24
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 16

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	43,978,205	43,978,225	TCTGGCTACACAGTCATGGTG
TUP	43,978,168	43,978,195	TGTTAATCAGGCCAAGAACAGCTTTGCA
TUR	43,978,132	43,978,153	CCAGTCAAGAGTGACAGAAGAC
TDF	43,969,764	43,969,788	GACATAACAGTAGCCCTGTCCTTAG
TDP	43,969,732	43,969,761	TGCCATCTACAGCTTCTTAGAGGACACCAA
TDR	43,969,702	43,969,722	TCAGGAGGAAACAGGTTCCAG

PCR Primers

Primer	Sequence
SU	CGCCATGGGAACGGCTGGTG
SD	GAAATGCCCCGAGTTCTAAC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	409 bp	-	+	+
TUF	TUR	94 bp	+	+	-
TDF	TDR	87 bp	+	+	-
NeoFwd	SD	392 bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
11230A-C3	VGB6	C57BL/6NTac
11230A-E8	VGB6	C57BL/6NTac
11230A-F9	VGB6	C57BL/6NTac
11230A-H7	VGB6	C57BL/6NTac