| Gene | Spaca5 |
| Chromosome | X |
| Deletion Start | 21,068,491 |
| Deletion End | 21,077,895 |
| Deletion Size | 9,405 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,068,498 | 21,068,520 | GCAGCATTGTGGTAGTGATCTTG |
| TUP | 21,068,522 | 21,068,551 | CCGTATTGTTGATTGCCAAATTGGATGCCA |
| TUR | 21,068,576 | 21,068,596 | CAGCCTCCTCCAGCTTCTTTG |
| TDF | 21,077,609 | 21,077,629 | CTGCTCAACCGCCATATTCTG |
| TDP | 21,077,636 | 21,077,662 | ATCATATGTGCCAAGAGGGTTGCATCC |
| TDR | 21,077,685 | 21,077,705 | GACCATGCCCACAAACTTACC |
PCR Primers
| Primer | Sequence |
| SU | CATATTTCTGAGGTTCTTCC |
| SD | TCTTGATTTCTATACCATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 391 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | 298 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11236A-A2 | VGB6 | C57BL/6NTac | |
| 11236A-F2 | VGB6 | C57BL/6NTac | |
