| Gene | Ttc30a2 |
| Chromosome | 2 |
| Deletion Start | 75,978,163 |
| Deletion End | 75,976,088 |
| Deletion Size | 2,076 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,977,659 | 75,977,681 | GCTCTACAAGGAGGGACACTATG |
| TUP | 75,977,632 | 75,977,658 | AAGCTGCCTGCTCCAAATTCTTTGCGG |
| TUR | 75,977,611 | 75,977,631 | GGTAGCCAGAAGCCTGCAAAG |
| TDF | 75,976,472 | 75,976,496 | AAACTATGACTTTGGCATCTCTCGA |
| TDP | 75,936,679 | 75,936,708 | TCAAAAGCCTGGAGCCTTACCATAAAAAGC |
| TDR | 75,976,277 | 75,976,301 | TTCTATGACAGCAGGGATGTTTCTG |
PCR Primers
| Primer | Sequence |
| SU | TTGACCTGTTGCTGTTCACTC |
| SD | GGTCCAGCAATTGGGTCTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 220 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11247A-A2 | VGB6 | C57BL/6NTac | |
| 11247A-E12 | VGB6 | C57BL/6NTac | |
| 11247A-D8 | VGB6 | C57BL/6NTac | |
