| Gene | Mfsd4 |
| Chromosome | 1 |
| Deletion Start | 132,067,889 |
| Deletion End | 132,038,894 |
| Deletion Size | 28,996 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 132,067,281 | 132,067,298 | GCTGGCCTGTGGTTTGAC |
| TUP | 132,067,255 | 132,067,279 | AGGAACCCACTGGTGATTCCACTCA |
| TUR | 132,067,196 | 132,067,215 | CCAGGAGCCTCACCATCTTG |
| TDF | 132,039,050 | 132,039,068 | GCATACAGCTTGCTTGCTC |
| TDP | 132,039,023 | 132,039,049 | TCAAGAGCTAAATGCTGCAGAGGAAGC |
| TDR | 132,038,950 | 132,038,971 | CAAGGGCTTTGTCTTGGGTAAG |
PCR Primers
| Primer | Sequence |
| SU | TCGCCAGGCTGAAAGGCTG |
| SD | TGGTGTGTGGAGAGGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 411 bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 119 bp | + | + | - |
| NeoFwd | SD | 304 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11248B-H12 | VGB6 | C57BL/6NTac | |
| 11248B-D2 | VGB6 | C57BL/6NTac | |
| 11248B-H2 | VGB6 | C57BL/6NTac | |
| 11248B-G8 | VGB6 | C57BL/6NTac | |
| 11248B-C10 | VGB6 | C57BL/6NTac | |
