| Gene | Mkrn3 |
| Chromosome | 7 |
| Deletion Start | 62,420,038 |
| Deletion End | 62,418,386 |
| Deletion Size | 1,653 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,419,751 | 62,419,770 | GCTGGACGAAGCAAATCCTC |
| TUP | 62,419,724 | 62,419,750 | TGCAGGTATTATCTGCATGGGCAGTGC |
| TUR | 62,419,692 | 62,419,714 | TCGTGAGAGTAGCGACAGTTATC |
| TDF | 62,418,965 | 62,418,988 | GAGGTGGTCTATGAGAAAGCTGAC |
| TDP | 62,418,939 | 62,418,961 | AGCGACCGCCGCTTTGGCATTCT |
| TDR | 62,418,896 | 62,418,917 | CCTGCGGATACACCTAAGACAG |
PCR Primers
| Primer | Sequence |
| SU | CATCCCAGTGTCTCAAGCAG |
| SD | GACTAGAAGTGATTGAATCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 391 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 369 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11253A-D5 | VGB6 | C57BL/6NTac | |
| 11253A-C3 | VGB6 | C57BL/6NTac | |
| 11253B-H5 | VGB6 | C57BL/6NTac | |
