| Gene | B3gnt8 |
| Chromosome | 7 |
| Deletion Start | 25,628,150 |
| Deletion End | 25,629,433 |
| Deletion Size | 1,284 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,628,709 | 25,628,730 | GATCACTGGTGACGTGGGAAAG |
| TUP | 25,628,731 | 25,628,754 | CCGGCGCTATGGTGACCTACTGCT |
| TUR | 25,628,768 | 25,628,788 | TGTCCGGTTGTAGGGAACATC |
| TDF | 25,628,947 | 25,628,966 | GATCTTCACCCAGGCCAAAC |
| TDP | 25,628,972 | 25,628,993 | CGCAAGCCCGGAGGACCCTTCT |
| TDR | 25,629,022 | 25,629,042 | CCACTCGCATAGGCTGGATAG |
PCR Primers
| Primer | Sequence |
| SU | GGTGTTCAGTCTAGGATAAC |
| SD | CAGCCTTGGCATGTATGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 349 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 340 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11257A-D9 | VGB6 | C57BL/6NTac | |
| 11257A-E7 | VGB6 | C57BL/6NTac | |
