| Gene | Kiss1r |
| Chromosome | 10 |
| Deletion Start | 79,918,677 |
| Deletion End | 79,922,089 |
| Deletion Size | 3,413 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,918,838 | 79,918,855 | TGGGCTGGTCGGAAACTC |
| TUP | 79,918,858 | 79,918,882 | TGGTCATCTACGTTATCTGCCGCCA |
| TUR | 79,918,907 | 79,918,928 | CACGCACTCACCGATGTAGAAG |
| TDF | 79,921,693 | 79,921,711 | TGGCACCCTCGAAGCTATG |
| TDP | 79,921,712 | 79,921,734 | CCGCCTACGCGGTCAAGATCTGG |
| TDR | 79,921,755 | 79,921,773 | CGGATTGAGCGCCGAGTTG |
PCR Primers
| Primer | Sequence |
| SU | GCTCTGAAGCGGCTACTACG |
| SD | GCAAAGCTAGACTTGGTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11258A-A9 | VGB6 | C57BL/6NTac | |
| 11258A-B6 | VGB6 | C57BL/6NTac | |
| 11258A-D10 | VGB6 | C57BL/6NTac | |
| 11258A-E5 | VGB6 | C57BL/6NTac | |
| 11258A-H10 | VGB6 | C57BL/6NTac | |
