| Gene | Prss23 |
| Chromosome | 7 |
| Deletion Start | 89,510,856 |
| Deletion End | 89,509,654 |
| Deletion Size | 1,203 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,510,722 | 89,510,741 | TGCCTCAGTCTACCCTCAAC |
| TUP | 89,510,697 | 89,510,720 | TAGCTAAGGCAGACTTCGACGCCA |
| TUR | 89,510,636 | 89,510,655 | GGCAGTGGTGTTCCCTTGTG |
| TDF | 89,510,170 | 89,510,188 | ACGCACCCATGTGCCCAAG |
| TDP | 89,510,142 | 89,510,166 | TGGATCAAGGGCAATGCCAATGACA |
| TDR | 89,510,112 | 89,510,133 | CCAGCAGGGCGTAGTCATAATC |
PCR Primers
| Primer | Sequence |
| SU | CCCTGTAATGTCCCATGGTC |
| SD | AAATTGCCTATTGAAGACTTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11263A-B10 | VGB6 | C57BL/6NTac | |
| 11263A-C7 | VGB6 | C57BL/6NTac | |
| 11263A-H8 | VGB6 | C57BL/6NTac | |
| 11263A-D10 | VGB6 | C57BL/6NTac | |
| 11263A-E9 | VGB6 | C57BL/6NTac | |
