| Gene | Mars2 |
| Chromosome | 1 |
| Deletion Start | 55,237,226 |
| Deletion End | 55,239,002 |
| Deletion Size | 1,777 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,238,029 | 55,238,046 | GACGACTCGCAGACCATC |
| TUP | 55,238,053 | 55,238,075 | TGGCTGGATGCCCTGGTCAACTA |
| TUR | 55,238,077 | 55,238,097 | TGGGTAGCCAACTACAGTGAG |
| TDF | 55,238,742 | 55,238,760 | CGTGCTTCACGTAGCCTTG |
| TDP | 55,238,764 | 55,238,788 | TGCTTGCGAGTGTTTGGAACTTTGC |
| TDR | 55,238,815 | 55,238,837 | CCCAGTCTTGACAGCAGTTTATC |
PCR Primers
| Primer | Sequence |
| SU | TTCCCCAACTGCCCTGACA |
| SD | AGTGTCAGCATCTTCCATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 356 bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 305 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11264A-F10 | VGB6 | C57BL/6NTac | |
| 11264A-D2 | VGB6 | C57BL/6NTac | |
