| Gene | Tceal1 |
| Chromosome | X |
| Deletion Start | 136,708,813 |
| Deletion End | 136,709,310 |
| Deletion Size | 498 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,708,887 | 136,708,908 | GCAGTCTCCTGAAAATCAGTGC |
| TUP | 136,708,909 | 136,708,934 | TCTGAGGAGGATCAATCCTCGGAGGA |
| TUR | 136,708,935 | 136,708,952 | TGCTCCTCGGAGGACAGA |
| TDF | 136,709,103 | 136,709,124 | GGGCAAACACAAGCTAGAGGAG |
| TDP | 136,709,132 | 136,709,157 | TCAAAGAAAGACTGGCCCGTATCCGC |
| TDR | 136,709,200 | 136,709,222 | TCCGCTGCCTGTATCATTTCTTC |
PCR Primers
| Primer | Sequence |
| SU | GAGCTACTGAGTGAGCTAAG |
| SD | ATGCAAATGGATCACACTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 120 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11265A-C9 | VGB6 | C57BL/6NTac | |
