| Gene | Uchl4 |
| Chromosome | 9 |
| Deletion Start | 64,235,225 |
| Deletion End | 64,236,305 |
| Deletion Size | 1,081 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 64,235,267 | 64,235,285 | AGGCCAACCCTGAGGTCAC |
| TUP | 64,235,289 | 64,235,315 | CCAGTTTCTCAAGCAATTAGGCCTGCA |
| TUR | 64,235,370 | 64,235,390 | GCGCACACTGGTCTTGGTATG |
| TDF | 64,235,838 | 64,235,862 | CGATGAGACGTTGTTAGAGGATGTC |
| TDP | 64,235,872 | 64,235,897 | TGCAAGAAGTTCATGGAACGTGACCC |
| TDR | 64,235,924 | 64,235,944 | GATGCTATGCTGCCGAGAGAG |
PCR Primers
| Primer | Sequence |
| SU | GTGACTGGAGAGCAAGCTG |
| SD | AGCCACCTGCCTGCTGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 380 bp | - | + | + |
| TUF | TUR | 124 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | 315 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11268A-B7 | VGB6 | C57BL/6NTac | |
| 11268A-C10 | VGB6 | C57BL/6NTac | |
| 11268A-F3 | VGB6 | C57BL/6NTac | |
| 11268A-H6 | VGB6 | C57BL/6NTac | |
