| Gene | Wfdc15a |
| Chromosome | 2 |
| Deletion Start | 164,200,033 |
| Deletion End | 164,199,586 |
| Deletion Size | 448 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,199,995 | 164,200,019 | CCTACTGTTCACAACAACCATCCTC |
| TUP | 164,199,973 | 164,199,994 | CTTTGCCTCAGCATGGCGCAGC |
| TUR | 164,199,936 | 164,199,959 | GACCATTCTGCATCACCTCCTTTC |
| TDF | 164,199,773 | 164,199,793 | CCGGAGTTTCTTCTTGACTGC |
| TDP | 164,199,746 | 164,199,772 | CCGTTTGTCCTGTTACCCGTATGCAGC |
| TDR | 164,199,725 | 164,199,744 | GCCCTTGCACCCTTTATCAC |
PCR Primers
| Primer | Sequence |
| SU | CAGGATAAGTGTGGGTTGGC |
| SD | TTCAACCATGAATTATGATCTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11270A-A1 | VGB6 | C57BL/6NTac | |
| 11270A-G11 | VGB6 | C57BL/6NTac | |
| 11270A-B7 | VGB6 | C57BL/6NTac | |
| 11270A-C4 | VGB6 | C57BL/6NTac | |
| 11270A-E4 | VGB6 | C57BL/6NTac | |
