| Gene | Ndst3 |
| Chromosome | 3 |
| Deletion Start | 123,672,318 |
| Deletion End | 123,671,347 |
| Deletion Size | 972 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,672,160 | 123,672,179 | CGGAAGTGGACTGTGGTGAC |
| TUP | 123,672,135 | 123,672,158 | TCCAGCACATACCATCCAGGCTGA |
| TUR | 123,672,097 | 123,672,121 | CCTTGAAGCATCGGAAATCATTGTC |
| TDF | 123,671,467 | 123,671,489 | TCTGGCTACACAAGCTCATCTTC |
| TDP | 123,671,441 | 123,671,464 | AGACGCCATCTCCTTCTTGTCGGG |
| TDR | 123,671,419 | 123,671,440 | CAAGGACAGTGTCAGCCTCTTC |
PCR Primers
| Primer | Sequence |
| SU | CACATGTGCTCCTCCACAAC |
| SD | ACAGATAGGATATCGTTGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11275B-D8 | VGB6 | C57BL/6NTac | |
| 11275B-G3 | VGB6 | C57BL/6NTac | |
| 11275D-F2 | VGB6 | C57BL/6NTac | |
| 11275C-F7 | VGB6 | C57BL/6NTac | |
