| Gene | Leng9 |
| Chromosome | 7 |
| Deletion Start | 4,149,672 |
| Deletion End | 4,148,210 |
| Deletion Size | 1,463 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,149,193 | 4,149,212 | GGGACGAGCATTGGACAGAG |
| TUP | 4,149,169 | 4,149,191 | TCACGGCAGAAATTCCCGACACA |
| TUR | 4,149,119 | 4,149,138 | GCAAGGGCATCCTGGGTATC |
| TDF | 4,148,719 | 4,148,739 | GTGACAGAGTCTGGGCTTAGG |
| TDP | 4,148,692 | 4,148,717 | CTGAGGTGGTCAAGGCCCAAGAGCAT |
| TDR | 4,148,654 | 4,148,675 | ACCAGGAACTCAGCACAAGAAG |
PCR Primers
| Primer | Sequence |
| SU | ACACATAAATTGGCTGGTTGG |
| SD | TACCCTCCCTTCTTTCCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11277A-H2 | VGB6 | C57BL/6NTac | |
| 11277A-C9 | VGB6 | C57BL/6NTac | |
| 11277A-E1 | VGB6 | C57BL/6NTac | |
| 11277A-G10 | VGB6 | C57BL/6NTac | |
| 11277A-F9 | VGB6 | C57BL/6NTac | |
| 11277A-B7 | VGB6 | C57BL/6NTac | |
