| Gene | Pramel7 |
| Chromosome | 2 |
| Deletion Start | 87,492,415 |
| Deletion End | 87,489,452 |
| Deletion Size | 2,964 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,492,358 | 87,492,378 | GGAGCCTGGTGAGGAATGAAG |
| TUP | 87,492,332 | 87,492,356 | ATTGACCGTCTCTGATGCGGAGTGC |
| TUR | 87,492,286 | 87,492,305 | GAGTGCTGGCCTCCTTGAAC |
| TDF | 87,489,837 | 87,489,858 | TCTCACTTCAGGACCCTTCTTC |
| TDP | 87,489,812 | 87,489,835 | TGCCCTGATCAAATGCACCCAGCT |
| TDR | 87,489,773 | 87,489,796 | TCCTCGGAGATGTCATTGTCATAC |
PCR Primers
| Primer | Sequence |
| SU | CAGGCAGGATCAATTTGTTG |
| SD | GATTTGGCTTGGCATACATTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11283A-E2 | VGB6 | C57BL/6NTac | |
| 11283A-G4 | VGB6 | C57BL/6NTac | |
| 11283A-G7 | VGB6 | C57BL/6NTac | |
| 11283A-H2 | VGB6 | C57BL/6NTac | |
| 11283A-G12 | VGB6 | C57BL/6NTac | |
| 11283A-H3 | VGB6 | C57BL/6NTac | |
