| Gene | G0s2 |
| Chromosome | 1 |
| Deletion Start | 193,272,771 |
| Deletion End | 193,272,443 |
| Deletion Size | 329 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 193,272,721 | 193,272,742 | CAAGGAGATGATGGCGCAGAAG |
| TUP | 193,272,692 | 193,272,719 | CCCGAGGGAAGCTAGTGAAGCTATACGT |
| TUR | 193,272,647 | 193,272,665 | ACTAGGCCGAGCACCACAC |
| TDF | 193,272,561 | 193,272,579 | CTGCGGGAAGCGTGTGAAC |
| TDP | 193,272,538 | 193,272,560 | AGCAGTCCCTCCACAAGCAGGCC |
| TDR | 193,272,511 | 193,272,530 | CTCCTGTGCCTTGCCTCCTG |
PCR Primers
| Primer | Sequence |
| SU | TGGGCAAGAGACACCAGAC |
| SD | ATCACAGTGCGTGCTGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 303 bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 238 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11287A-G11 | VGB6 | C57BL/6NTac | |
| 11287A-F11 | VGB6 | C57BL/6NTac | |
| 11287A-G1 | VGB6 | C57BL/6NTac | |
| 11287A-D11 | VGB6 | C57BL/6NTac | |
