| Gene | Ubqlnl |
| Chromosome | 7 |
| Deletion Start | 104,150,285 |
| Deletion End | 104,148,438 |
| Deletion Size | 1,848 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,149,950 | 104,149,971 | CAGATCCCTGGCTCACTCTTTC |
| TUP | 104,149,918 | 104,149,945 | ACCTGGTAACCAACAATCCCTGCCATCA |
| TUR | 104,149,881 | 104,149,901 | GCACACCATGCTGCTATTTCC |
| TDF | 104,149,188 | 104,149,206 | CCAGGGCCAAAGCAATGTC |
| TDP | 104,149,155 | 104,149,178 | CAGCAGCCAGCTGAGATTCCAGTC |
| TDR | 104,149,126 | 104,149,150 | GGCTTCTGGTTGATTGATATGGTTG |
PCR Primers
| Primer | Sequence |
| SU | AGTATGAGTCAGTAGCCACG |
| SD | CAATAAAATGAAGGCAAGCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 377 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 306 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11289A-D6 | VGB6 | C57BL/6NTac | |
| 11289A-D8 | VGB6 | C57BL/6NTac | |
| 11289A-E9 | VGB6 | C57BL/6NTac | |
| 11289A-E7 | VGB6 | C57BL/6NTac | |
| 11289A-E3 | VGB6 | C57BL/6NTac | |
| 11289A-H3 | VGB6 | C57BL/6NTac | |
