| Gene | Pdxp |
| Chromosome | 15 |
| Deletion Start | 78,913,956 |
| Deletion End | 78,918,493 |
| Deletion Size | 4,538 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,914,665 | 78,914,687 | AGGGAATTGCAGGTGGGATACAG |
| TUP | 78,914,689 | 78,914,712 | TTTGGAACCGGCCTCTTGGGCTTT |
| TUR | 78,914,765 | 78,914,784 | AGCTCTCCACCGGCATCATC |
| TDF | 78,917,745 | 78,917,764 | TGACTAGGTCAGAGGTGTGG |
| TDP | 78,917,766 | 78,917,790 | CATTTCTGTGCCCTGCTGCATTCCA |
| TDR | 78,917,831 | 78,917,851 | GGTGGTCAGTGCTCCAAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CTCTTCATTGGTCGCCTCC |
| SD | AGAGTGGGAGGAACTTAAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 337 bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | 359 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11291A-C12 | VGB6 | C57BL/6NTac | |
| 11291A-D10 | VGB6 | C57BL/6NTac | |
| 11291A-D6 | VGB6 | C57BL/6NTac | |
