| Gene | Magee2 |
| Chromosome | X |
| Deletion Start | 104,857,040 |
| Deletion End | 104,855,460 |
| Deletion Size | 1,581 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,856,958 | 104,856,977 | CAGGGTGAAATGCAGGCTAC |
| TUP | 104,856,937 | 104,856,957 | TAATGCCTCCGGGCCTGCCAC |
| TUR | 104,856,880 | 104,856,900 | CCCTGTGGATCGTTTGGAATC |
| TDF | 104,855,808 | 104,855,829 | GCCAATCCTGGGTTTGATCTTC |
| TDP | 104,855,781 | 104,855,803 | TGGGCAACCGTGTCAAAGAGGCC |
| TDR | 104,855,714 | 104,855,736 | GCTTACAGGTGATGGCATGTTTC |
PCR Primers
| Primer | Sequence |
| SU | AGGATGCGGCAAAGCTAGG |
| SD | TCTTATACTGAAACAAGACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 345 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 116 bp | + | + | - |
| NeoFwd | SD | 324 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11295A-A2 | VGB6 | C57BL/6NTac | |
| 11295A-A5 | VGB6 | C57BL/6NTac | |
| 11295A-F12 | VGB6 | C57BL/6NTac | |
